Highly diverse TCRα chain repertoire of pre-immune CD8(+) T cells reveals new insights in gene recombination.

Détails

ID Serval
serval:BIB_2B947DF61C00
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Highly diverse TCRα chain repertoire of pre-immune CD8(+) T cells reveals new insights in gene recombination.
Périodique
Embo Journal
Auteur(s)
Genolet R., Stevenson B.J., Farinelli L., Osterås M., Luescher I.F.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
31
Numéro
7
Pages
1666-1678
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Although the T-cell receptor αδ (TCRαδ) locus harbours large libraries of variable (TRAV) and junctional (TRAJ) gene segments, according to previous studies the TCRα chain repertoire is of limited diversity due to restrictions imposed by sequential coordinate TRAV-TRAJ recombinations. By sequencing tens of millions of TCRα chain transcripts from naive mouse CD8(+) T cells, we observed a hugely diverse repertoire, comprising nearly all possible TRAV-TRAJ combinations. Our findings are not compatible with sequential coordinate gene recombination, but rather with a model in which contraction and DNA looping in the TCRαδ locus provide equal access to TRAV and TRAJ gene segments, similarly to that demonstrated for IgH gene recombination. Generation of the observed highly diverse TCRα chain repertoire necessitates deletion of failed attempts by thymic-positive selection and is essential for the formation of highly diverse TCRαβ repertoires, capable of providing good protective immunity.
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/05/2012 16:33
Dernière modification de la notice
08/05/2019 16:21
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