Comparative study and identification of potent eukaryotic transcriptional repressors in gene switch systems.

Détails

ID Serval
serval:BIB_2B92BDC0FC80
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative study and identification of potent eukaryotic transcriptional repressors in gene switch systems.
Périodique
Journal of Biotechnology
Auteur(s)
Imhof M.O., Chatellard P., Mermod N.
ISSN
0168-1656[print], 0168-1656[linking]
Statut éditorial
Publié
Date de publication
2002
Volume
97
Numéro
3
Pages
275-285
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In mammalian cells, proper gene regulation is achieved by the complex interplay of transcription factors that activate or repress gene expression by binding to the regulatory regions of target promoters. While transcriptional activators have been extensively characterised and classified into functional groups, relatively little is known about the comparative strength and cell type-specificity of transcriptional repressors. Here, we have compared the ability of a series of eukaryotic repression domains to silence basal and activated transcription. A series of the most potent repression domains was further tested in the context of a gene therapy gene-switch system in various cell types. The results indicate that the analysed repression domains exert varying silencing activities in different promoter contexts. Furthermore, their potential for gene silencing varies also depending on the cellular context. When multimerised within one chimeric repressor protein, particular combinations of repressor domains were found to display synergistic repressing effects and efficient repression in a panel of cell lines. This approach thus allowed the identification of transcriptional repressors that are both potent and versatile in terms of cellular specificity as a basis for gene switch systems.
Mots-clé
Cell Line, DNA, Bacterial/genetics, Eukaryotic Cells/physiology, Gene Expression/genetics, Gene Expression Regulation/genetics, Gene Transfer Techniques, Humans, Models, Genetic, Promoter Regions, Genetic, Repressor Proteins/genetics, Repressor Proteins/metabolism, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 10:41
Dernière modification de la notice
20/08/2019 13:10
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