GC-MS Based Metabolomics and NMR Spectroscopy Investigation of Food Intake Biomarkers for Milk and Cheese in Serum of Healthy Humans.

Détails

Ressource 1Télécharger: 29570652.pdf (27540.36 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_2B78DE188FA1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
GC-MS Based Metabolomics and NMR Spectroscopy Investigation of Food Intake Biomarkers for Milk and Cheese in Serum of Healthy Humans.
Périodique
Metabolites
Auteur(s)
Trimigno A., Münger L., Picone G., Freiburghaus C., Pimentel G., Vionnet N., Pralong F., Capozzi F., Badertscher R., Vergères G.
ISSN
2218-1989 (Print)
ISSN-L
2218-1989
Statut éditorial
Publié
Date de publication
23/03/2018
Peer-reviewed
Oui
Volume
8
Numéro
2
Pages
NA
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The identification and validation of food intake biomarkers (FIBs) in human biofluids is a key objective for the evaluation of dietary intake. We report here the analysis of the GC-MS and 1H-NMR metabolomes of serum samples from a randomized cross-over study in 11 healthy volunteers having consumed isocaloric amounts of milk, cheese, and a soy drink as non-dairy alternative. Serum was collected at baseline, postprandially up to 6 h, and 24 h after consumption. A multivariate analysis of the untargeted serum metabolomes, combined with a targeted analysis of candidate FIBs previously reported in urine samples from the same study, identified galactitol, galactonate, and galactono-1,5-lactone (milk), 3-phenyllactic acid (cheese), and pinitol (soy drink) as candidate FIBs for these products. Serum metabolites not previously identified in the urine samples, e.g., 3-hydroxyisobutyrate after cheese intake, were detected. Finally, an analysis of the postprandial behavior of candidate FIBs, in particular the dairy fatty acids pentadecanoic acid and heptadecanoic acid, revealed specific kinetic patterns of relevance to their detection in future validation studies. Taken together, promising candidate FIBs for dairy intake appear to be lactose and metabolites thereof, for lactose-containing products, and microbial metabolites derived from amino acids, for fermented dairy products such as cheese.
Mots-clé
biomarker, cheese, metabolomics, milk, nutrition, serum metabolome, soy drink
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/03/2018 20:22
Dernière modification de la notice
08/05/2019 16:20
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