Article: article from journal or magazin.
A monoclonal antibody which blocks the function of factor D of human complement.
Journal of immunological methods
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Factor D is an essential enzyme for activation of complement by the alternative pathway (AP). It has been difficult to obtain mouse monoclonal antibodies (Mabs) which block the function of factor D. We have developed a strategy to obtain such Mabs using a double screening procedure of the initial clones. We selected the clone whose supernatant had the lowest level of anti-factor D Ab by ELISA and abolished factor D haemolytic activity. Addition of this Mab to human serum was shown to abolish conversion of C3 by cobra venom factor, haemolysis of rabbit erythrocytes, and activation of C3 and C5 by cuprophane dialysis membranes.
Animals, Antibodies, Monoclonal, Complement Activating Enzymes, Complement Activation, Complement C3a, Complement C5a, Complement Factor D, Complement Pathway, Alternative, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Inbred BALB C, Rabbits
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