Red blood cell structure and dynamics explored with digital holographic microspcopy

Détails

ID Serval
serval:BIB_2B585D793299
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Red blood cell structure and dynamics explored with digital holographic microspcopy
Périodique
Progress in Biomedical Optics and Imaging
Auteur(s)
Marquet P., Rappaz B., Barbuld A., Korensteind R., Depeursinge C., Magistretti P.
ISSN
0277-786X
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
7182
Pages
71821A
Langue
anglais
Résumé
Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. Thanks to a decoupling procedure cell thickness and intracellular refractive index can be measured separately. Consequently, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) on the whole cell surface.
©2009 COPYRIGHT SPIE--The International Society for Optical Engineering.
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Création de la notice
22/01/2010 13:45
Dernière modification de la notice
20/08/2019 13:10
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