Tissue-specific and SRSF1-dependent splicing of fibronectin, a matrix protein that controls host cell invasion.

Détails

Ressource 1Télécharger: BIB_2AD6B0F09199.P001.pdf (5979.86 [Ko])
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_2AD6B0F09199
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Tissue-specific and SRSF1-dependent splicing of fibronectin, a matrix protein that controls host cell invasion.
Périodique
Molecular Biology of the Cell
Auteur(s)
Lopez-Mejia I.C., De Toledo M., Della Seta F., Fafet P., Rebouissou C., Deleuze V., Blanchard J.M., Jorgensen C., Tazi J., Vignais M.L.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
24
Numéro
20
Pages
3164-3176
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Cell invasion targets specific tissues in physiological placental implantation and pathological metastasis, which raises questions about how this process is controlled. We compare dermis and endometrium capacities to support trophoblast invasion, using matching sets of human primary fibroblasts in a coculture assay with human placental explants. Substituting endometrium, the natural trophoblast target, with dermis dramatically reduces trophoblast interstitial invasion. Our data reveal that endometrium expresses a higher rate of the fibronectin (FN) extra type III domain A+ (EDA+) splicing isoform, which displays stronger matrix incorporation capacity. We demonstrate that the high FN content of the endometrium matrix, and not specifically the EDA domain, supports trophoblast invasion by showing that forced incorporation of plasma FN (EDA-) promotes efficient trophoblast invasion. We further show that the serine/arginine-rich protein serine/arginine-rich splicing factor 1 (SRSF1) is more highly expressed in endometrium and, using RNA interference, that it is involved in the higher EDA exon inclusion rate in endometrium. Our data therefore show a mechanism by which tissues can be distinguished, for their capacity to support invasion, by their different rates of EDA inclusion, linked to their SRSF1 protein levels. In the broader context of cancer pathology, the results suggest that SRSF1 might play a central role not only in the tumor cells, but also in the surrounding stroma.
Pubmed
Web of science
Création de la notice
12/01/2014 17:56
Dernière modification de la notice
03/03/2018 15:23
Données d'usage