Molecular Epidemiology of Human Rhinoviruses and Enteroviruses Highlights Their Diversity in Sub-Saharan Africa.

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Version: author
Serval ID
serval:BIB_2A4C1360E747
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular Epidemiology of Human Rhinoviruses and Enteroviruses Highlights Their Diversity in Sub-Saharan Africa.
Journal
Viruses
Author(s)
L'Huillier A.G., Kaiser L., Petty T.J., Kilowoko M., Kyungu E., Hongoa P., Vieille G., Turin L., Genton B., D'Acremont V., Tapparel C.
ISSN
1999-4915 (Electronic)
ISSN-L
1999-4915
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
7
Number
12
Pages
6412-6423
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Human rhinoviruses (HRVs) and enteroviruses (HEVs) belong to the Enterovirus genus and are the most frequent cause of infection worldwide, but data on their molecular epidemiology in Africa are scarce. To understand HRV and HEV molecular epidemiology in this setting, we enrolled febrile pediatric patients participating in a large prospective cohort assessing the causes of fever in Tanzanian children. Naso/oropharyngeal swabs were systematically collected and tested by real-time RT-PCR for HRV and HEV. Viruses from positive samples were sequenced and phylogenetic analyses were then applied to highlight the HRV and HEV types as well as recombinant or divergent strains. Thirty-eight percent (378/1005) of the enrolled children harboured an HRV or HEV infection. Although some types were predominant, many distinct types were co-circulating, including a vaccinal poliovirus, HEV-A71 and HEV-D68. Three HRV-A recombinants were identified: HRV-A36/HRV-A67, HRV-A12/HRV-A67 and HRV-A96/HRV-A61. Four divergent HRV strains were also identified: one HRV-B strain and three HRV-C strains. This is the first prospective study focused on HRV and HEV molecular epidemiology in sub-Saharan Africa. This systematic and thorough large screening with careful clinical data management confirms the wide genomic diversity of these viruses, brings new insights about their evolution and provides data about associated symptoms.
Keywords
Enterovirus/classification, Enterovirus/genetics, Enterovirus Infections/epidemiology, Enterovirus Infections/virology, Genetic Variation, Genotype, Humans, Molecular Epidemiology, Nasopharynx/virology, Oropharynx/virology, Phylogeny, Picornaviridae Infections/epidemiology, Picornaviridae Infections/virology, Prevalence, Prospective Studies, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Rhinovirus/classification, Rhinovirus/genetics, Sequence Analysis, DNA, Tanzania/epidemiology
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2016 17:56
Last modification date
20/08/2019 13:09
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