Suitability of 3D human brain spheroid models to distinguish toxic effects of gold and poly-lactic acid nanoparticles to assess biocompatibility for brain drug delivery.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_2A2AD5708646
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Suitability of 3D human brain spheroid models to distinguish toxic effects of gold and poly-lactic acid nanoparticles to assess biocompatibility for brain drug delivery.
Journal
Particle and fibre toxicology
Author(s)
Leite PEC, Pereira M.R., Harris G., Pamies D., Dos Santos LMG, Granjeiro J.M., Hogberg H.T., Hartung T., Smirnova L.
ISSN
1743-8977 (Electronic)
ISSN-L
1743-8977
Publication state
Published
Issued date
03/06/2019
Peer-reviewed
Oui
Volume
16
Number
1
Pages
22
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The blood brain barrier (BBB) is the bottleneck of brain-targeted drug development. Due to their physico-chemical properties, nanoparticles (NP) can cross the BBB and accumulate in different areas of the central nervous system (CNS), thus are potential tools to carry drugs and treat brain disorders. In vitro systems and animal models have demonstrated that some NP types promote neurotoxic effects such as neuroinflammation and neurodegeneration in the CNS. Thus, risk assessment of the NP is required, but current 2D cell cultures fail to mimic complex in vivo cellular interactions, while animal models do not necessarily reflect human effects due to physiological and species differences.
We evaluated the suitability of in vitro models that mimic the human CNS physiology, studying the effects of metallic gold NP (AuNP) functionalized with sodium citrate (Au-SC), or polyethylene glycol (Au-PEG), and polymeric polylactic acid NP (PLA-NP). Two different 3D neural models were used (i) human dopaminergic neurons differentiated from the LUHMES cell line (3D LUHMES) and (ii) human iPSC-derived brain spheroids (BrainSpheres). We evaluated NP uptake, mitochondrial membrane potential, viability, morphology, secretion of cytokines, chemokines and growth factors, and expression of genes related to ROS regulation after 24 and 72 h exposures. NP were efficiently taken up by spheroids, especially when PEGylated and in presence of glia. AuNP, especially PEGylated AuNP, effected mitochondria and anti-oxidative defense. PLA-NP were slightly cytotoxic to 3D LUHMES with no effects to BrainSpheres.
3D brain models, both monocellular and multicellular are useful in studying NP neurotoxicity and can help identify how specific cell types of CNS are affected by NP.
Keywords
3D LUHMES, CNS, Drug delivery, Nanoparticle, iPSC-derived BrainSpheres
Pubmed
Web of science
Open Access
Yes
Create date
17/06/2019 16:39
Last modification date
15/01/2021 7:08
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