An isoelectric focusing method for the study of the humoral response against the antigen 85 complex of Mycobacterium bovis BCG in the different forms of leprosy.
Details
Serval ID
serval:BIB_299FAC994FEC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An isoelectric focusing method for the study of the humoral response against the antigen 85 complex of Mycobacterium bovis BCG in the different forms of leprosy.
Journal
Journal of Immunological Methods
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Publication state
Published
Issued date
1991
Volume
145
Number
1-2
Pages
223-228
Language
english
Abstract
Isoelectric focusing was used to separate the three components of the antigen 85 complex of Mycobacterium bovis BCG. Antibody responses of leprosy patients against each Mycobacterium bovis BCG. Antibody responses of leprosy patients against each component were quantitated by densitometric analysis of immunoblot assays. The 85A component was recognized by 40% (8/20) of the lepromin positive and negative healthy subjects, by 76% (19/25) of the tuberculoid and by 96% (24/25) of the lepromatous leprosy sera. In contrast, the 85B component was not stained by the control sera, nor by the tuberculoid leprosy sera but by 64% (16/25) of the lepromatous leprosy sera. The results suggest that antigen 85B contains one or several epitopes that are specifically recognized by sera of lepromatous leprosy patients only.
Keywords
Antibodies, Bacterial/biosynthesis, Antigens, Bacterial/chemistry, Antigens, Bacterial/immunology, Blotting, Western, Humans, Isoelectric Point, Lepromin/analysis, Leprosy/diagnosis, Leprosy/immunology, Leprosy, Lepromatous/diagnosis, Leprosy, Tuberculoid/diagnosis, Mycobacterium bovis/immunology
Pubmed
Web of science
Create date
28/01/2008 12:06
Last modification date
20/08/2019 14:09
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