Carbon monoxide analysis method in human blood by Airtight Gas Syringe - Gas Chromatography - Mass Spectrometry (AGS-GC-MS): Relevance for postmortem poisoning diagnosis.

Détails

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Etat: Serval
Version: Author's accepted manuscript
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_2939CA1FEC9B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Carbon monoxide analysis method in human blood by Airtight Gas Syringe - Gas Chromatography - Mass Spectrometry (AGS-GC-MS): Relevance for postmortem poisoning diagnosis.
Périodique
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Auteur(s)
Oliverio S., Varlet V.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Statut éditorial
Publié
Date de publication
15/07/2018
Peer-reviewed
Oui
Volume
1090
Pages
81-89
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Carbon monoxide is one of the most abundant toxic air pollutants. Symptoms of a CO intoxication are non-specific, leading to a high number of misdiagnosed CO poisoning cases that are missing in the disease statistics. The chemical nature of the molecule makes it difficult to detect for long periods and at low levels, thus requiring a very accurate and sensitive method. Current methods capable of accurate and sensitive analyses are available, however an inconsistency between results and symptoms are frequently reported. Therefore, an improved method for the analysis of carbon monoxide in blood and in the headspace (HS) of the sampling tube with the use of Airtight Gas Syringe - Gas Chromatography - Mass Spectrometry (AGS-GC-MS) is hereby presented and validated, for CO concentrations in a range of 10-200 nmol/mL HS (2-40 μmol/mL blood). Analytical LOQ is found at 0.9 nmol/mL HS (0.18 μmol/mL blood) and LOD at 0.1 nmol/mL gas. Application to intoxicated samples from autopsies and comparison to previously published methods show that this method is more appropriate, since performed under fully controlled conditions. Results show higher CO concentrations compared to previous approaches, indicating that results might have been underestimating the true blood CO burden. Therefore, this approach has the potential to help reduce the misdiagnosed cases and the gap between measurement and diagnosis of CO poisonings.
Mots-clé
Adult, Aged, Autopsy, Blood Gas Analysis/methods, Carbon Monoxide/blood, Carboxyhemoglobin/analysis, Female, Forensic Toxicology, Gas Chromatography-Mass Spectrometry/methods, Humans, Limit of Detection, Linear Models, Male, Reproducibility of Results, Young Adult, Airtight Gas Syringe, Analytical measurement, Carbon monoxide, Carboxyhaemoglobin, GC-MS
Pubmed
Web of science
Création de la notice
31/05/2018 19:05
Dernière modification de la notice
13/07/2019 16:42
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