Quantitative proteomics of rat livers shows that unrestricted feeding is stressful for proteostasis with implications on life span.

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_290FCC29088F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantitative proteomics of rat livers shows that unrestricted feeding is stressful for proteostasis with implications on life span.
Périodique
Aging
Auteur(s)
Gat-Yablonski G., Finka A., Pinto G., Quadroni M., Shtaif B., Goloubinoff P.
ISSN
1945-4589 (Electronic)
ISSN-L
1945-4589
Statut éditorial
Publié
Date de publication
08/2016
Peer-reviewed
Oui
Volume
8
Numéro
8
Pages
1735-1758
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Studies in young mammals on the molecular effects of food restriction leading to prolong adult life are scares. Here, we used high-throughput quantitative proteomic analysis of whole rat livers to address the molecular basis for growth arrest and the apparent life-prolonging phenotype of the food restriction regimen. Over 1800 common proteins were significantly quantified in livers of ad libitum, restriction- and re-fed rats, which summed up into 92% of the total protein mass of the cells. Compared to restriction, ad libitum cells contained significantly less mitochondrial catabolic enzymes and more cytosolic and ER HSP90 and HSP70 chaperones, which are hallmarks of heat- and chemically-stressed tissues. Following re-feeding, levels of HSPs nearly reached ad libitum levels. The quantitative and qualitative protein values indicated that the restriction regimen was a least stressful condition that used minimal amounts of HSP-chaperones to maintain optimal protein homeostasis and sustain optimal life span. In contrast, the elevated levels of HSP-chaperones in ad libitum tissues were characteristic of a chronic stress, which in the long term could lead to early aging and shorter life span.

Mots-clé
Aging/metabolism, Animals, Eating/physiology, Feeding Behavior/physiology, Food Deprivation, HSP70 Heat-Shock Proteins/metabolism, HSP90 Heat-Shock Proteins/metabolism, Homeostasis/physiology, Liver/metabolism, Longevity/physiology, Proteomics, Rats, aging, catch up growth, food restriction, heat shock proteins, mitochondria
Pubmed
Web of science
Création de la notice
16/09/2016 19:17
Dernière modification de la notice
20/08/2019 14:08
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