Article: article from journal or magazin.
Calcitonin measurement on fine needle washouts: preanalytical issues and normal reference values.
Publication types: Journal ArticlePublication Status: ppublish
Calcitonin (CT) measurement on fine-needle aspiration cytology (FNAC) washouts (FNAC-CT) is a promising tool in the diagnosis of medullary thyroid carcinoma (MTC). Guidelines for the method with codified cut-off are needed to use this technique in clinical routine. This study was undertaken to assess the best pre-analytical procedure and to define a reliable reference value for FNAC-CT. Fifty-four patients underwent thyroid surgery, so MTC was excluded by surgical pathology examination and CT immunostains. Before surgery, FNAC-CT was measured on 78 thyroid nodules from such 54 patients. Needles were rinsed by normal saline and specific CT-free dilution buffer, and CT was measured by a fully automated immunochemiluminometric assay. FNAC-CT levels were not significantly different in normal saline or specific buffer. The calculated 97.5th upper FNAC-CT value was 8.5 pg/mL (saline) and 7.43 pg/mL (buffer), respectively. Seeing its relatively large sample size, rigorous selection criteria and inclusion of CT immunostaining of thyroid nodules, the present study provides a reliable guideline and a clinically relevant reference value for FNAC-CT measurement in thyroid nodules.
Adenocarcinoma, Follicular/blood, Adenocarcinoma, Follicular/chemistry, Biopsy, Fine-Needle, Calcitonin/analysis, Carcinoma/blood, Carcinoma/chemistry, Carcinoma, Medullary/blood, Carcinoma, Medullary/chemistry, Female, Goiter, Nodular/blood, Goiter, Nodular/pathology, Humans, Limit of Detection, Male, Reference Values, Sodium Chloride/chemistry, Solubility, Thyroid Neoplasms/blood, Thyroid Neoplasms/chemistry, Thyroid Nodule/blood, Thyroid Nodule/chemistry, Thyroidectomy
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