Interleukin-4 signaling in B lymphocytes from patients with X-linked severe combined immunodeficiency

Détails

ID Serval
serval:BIB_2894D0AF945E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Interleukin-4 signaling in B lymphocytes from patients with X-linked severe combined immunodeficiency
Périodique
J Biol Chem
Auteur(s)
Taylor N., Candotti F., Smith S., Oakes S. A., Jahn T., Isakov J., Puck J. M., O'Shea J. J., Weinberg K., Johnston J. A.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1997
Volume
272
Numéro
11
Pages
7314-9
Langue
anglais
Notes
Taylor, N
Candotti, F
Smith, S
Oakes, S A
Jahn, T
Isakov, J
Puck, J M
O'Shea, J J
Weinberg, K
Johnston, J A
eng
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
J Biol Chem. 1997 Mar 14;272(11):7314-9.
Résumé
Interleukin-4 (IL-4) is an important cytokine for B and T lymphocyte function and mediates its effects via a receptor that contains gammac. B cells derived from patients with X-linked severe combined immunodeficiency (X-SCID) are deficient in gammac and provide a useful model in which to dissect the role of this subunit in IL-4-mediated signaling. We found that although IL-4 stimulation of X-SCID B cells did not result in Janus tyrosine kinase-3 (JAK3) phosphorylation, other IL-4 substrates including JAK1 and IRS-1 were phosphorylated. Additionally, we detected signal transducers and activators of transcription 6 (STAT6) tyrosine phosphorylation and DNA binding activity in X-SCID B cells with a wide range of gammac mutations. However, reconstitution of these X-SCID B cells with gammac enhanced IL-4-mediated responses including STAT6 phosphorylation and DNA binding activity and resulted in increased CD23 expression. Thus, gammac is not necessary to trigger IL-4-mediated responses in B cells, but its presence is important for optimal IL-4-signaling. These results suggest that two distinct IL-4 signaling pathways exist.
Mots-clé
B-Lymphocytes/*immunology/metabolism, Cells, Cultured, Humans, Interleukin-4/*immunology/metabolism, Male, Molecular Sequence Data, STAT6 Transcription Factor, Severe Combined Immunodeficiency/*immunology, *Signal Transduction, Trans-Activators/*metabolism
Pubmed
Création de la notice
01/11/2017 11:29
Dernière modification de la notice
20/08/2019 14:08
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