Non-neural regions of the adult human eye: a potential source of neurons?

Details

Serval ID
serval:BIB_28652
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Non-neural regions of the adult human eye: a potential source of neurons?
Journal
Investigative Ophthalmology and Visual Science
Author(s)
Arsenijevic Y., Taverney N., Kostic C., Tekaya M., Riva F., Zografos L., Schorderet D., Munier F.
ISSN
0146-0404
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
44
Number
2
Pages
799-807
Language
english
Abstract
PURPOSE. Because it is known that both melanocytes and neurons are generated from neural crest stem cells and their derived precursors, the current study was undertaken to evaluate whether adult human ocular tissues, containing melanocytes, have the capacity to generate neuronlike cells in vitro. METHODS. Choroid and Sclera cells from adult human eyes were separately dissociated and cultivated in the presence of epidermal growth factor and 10% fetal bovine serum. No retinal pigmented epithelial cells were detected. After cell growth, cells were transferred under conditions known to induce neuronal differentiation. Cells were plated on laminin in the presence of fibroblast growth factor-2 or brain-derived neurotrophic factor. RESULTS. Cells derived from the sclera and the choroid of 15 donors proliferated to attain a 10(8)-fold increase in the number of cells within 4 months. At each passage, groups of cells differentiated into cells with neuronal morphology, expressing neuronal markers confirmed by immunocytochemistry and RT-PCR analyses, such as beta-tubulin-III, neurofilament, and tau. Parallel to neuronlike formation, glialike cells, revealed by expression of vimentin and P0, were generated in large amounts. Although, absent from choroid and sclera tissues, nondifferentiated cells appeared in cultures. CONCLUSIONS. The adult human eye conserves cells able to recapitulate certain neural developmental features. This observation opens new perspectives to study human neurogenesis and to provide an important source of neurons for transplantation studies in the retina and other regions of the central nervous system.
Keywords
Adolescent, Adult, Aged, Aged, 80 and over, Biological Markers, Brain-Derived Neurotrophic Factor, Cell Differentiation, Cell Division, Cells, Cultured, Choroid, Epidermal Growth Factor, Fibroblast Growth Factor 2, Humans, Immunohistochemistry, Melanocytes, Middle Aged, Neuroglia, Neurons, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Sclera
Pubmed
Web of science
Create date
19/11/2007 12:25
Last modification date
20/08/2019 13:07
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