Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR.

Détails

Ressource 1Télécharger: BIB_27B0BF68F622.P001.pdf (1460.55 [Ko])
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_27B0BF68F622
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR.
Périodique
Bmc Research Notes
Auteur(s)
Stojanov M., Moreillon P., Sakwinska O.
ISSN
1756-0500 (Electronic)
ISSN-L
1756-0500
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
8
Pages
828
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: epublish
Résumé
BACKGROUND: Methicillin-resistance in staphylococci is conferred by the mecA gene, located on the genomic island Staphylococcal Cassette Chromosome mec (SCCmec). SCCmec mobility relies on the Ccr recombinases, which catalyze insertion and excision form the host's chromosome. Although being a crucial step in its horizontal transfer, little is known about the dynamics of SCCmec excision.
RESULTS: A quantitative PCR-based method was used to measure the rate of SCCmec excision by amplifying the chromosome-chromosome junction and the circularized SCCmec resulting from excision. SCCmec excision rate was measured in methicillin-resistant Staphylococcus aureus (MRSA) strain N315 at various growth times in broth cultures. In the present experimental settings, excision of SCCmec occurred at a rate of approximately 2 × 10(-6) in MRSA N315.
CONCLUSION: This work brings new insights in the poorly understood SCCmec excision process. The results presented herein suggest a model in which excision occurs during a limited period of time at the early stages of growth.
Mots-clé
Bacterial Proteins/genetics, Chromosomes, Bacterial/genetics, DNA, Bacterial/genetics, DNA, Bacterial/metabolism, DNA, Circular/genetics, DNA, Circular/metabolism, Genomic Islands/genetics, Methicillin Resistance/genetics, Methicillin-Resistant Staphylococcus aureus/genetics, Models, Genetic, Penicillin-Binding Proteins/genetics, Polymerase Chain Reaction/methods, Recombinases/metabolism
Pubmed
Open Access
Oui
Création de la notice
19/06/2016 17:58
Dernière modification de la notice
08/05/2019 16:05
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