Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae
Details
Serval ID
serval:BIB_27A26222D310
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae
Journal
Biochemical and Biophysical Research Communications
ISSN
0006-291X (Print)
Publication state
Published
Issued date
08/1987
Volume
146
Number
3
Pages
1311-7
Notes
Journal Article
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Aug 14
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Aug 14
Abstract
We have isolated the gene for cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.
Keywords
Amino Acid Sequence
Base Sequence
Candida/enzymology/*genetics
Cytochrome P-450 Enzyme System/*genetics
DNA, Fungal/genetics/isolation & purification
Drug Resistance
Escherichia coli/genetics
*Genes
*Genes, Fungal
Ketoconazole/pharmacology
Oxidoreductases/*genetics
Plasmids
Saccharomyces cerevisiae/drug effects/genetics
Pubmed
Web of science
Create date
25/01/2008 15:40
Last modification date
20/08/2019 14:06
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