Fluorine MR Imaging of Inflammation in Atherosclerotic Plaque in Vivo.

Détails

Ressource 1Télécharger: BIB_279183AA9412.P001.pdf (2291.39 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_279183AA9412
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fluorine MR Imaging of Inflammation in Atherosclerotic Plaque in Vivo.
Périodique
Radiology
Auteur(s)
van Heeswijk R.B., Pellegrin M., Flögel U., Gonzales C., Aubert J.F., Mazzolai L., Schwitter J., Stuber M.
ISSN
1527-1315 (Electronic)
ISSN-L
0033-8419
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
275
Numéro
2
Pages
421-429
Langue
anglais
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
PURPOSE: To preliminarily test the hypothesis that fluorine 19 ((19)F) magnetic resonance (MR) imaging enables the noninvasive in vivo identification of plaque inflammation in a mouse model of atherosclerosis, with histologic findings as the reference standard.
MATERIALS AND METHODS: The animal studies were approved by the local animal ethics committee. Perfluorocarbon (PFC) emulsions were injected intravenously in a mouse model of atherosclerosis (n = 13), after which (19)F and anatomic MR imaging were performed at the level of the thoracic aorta and its branches at 9.4 T. Four of these animals were imaged repeatedly (at 2-14 days) to determine the optimal detection time. Repeated-measures analysis of variance with a Tukey test was applied to determine if there was a significant change in (19)F signal-to-noise ratio (SNR) of the plaques and liver between the time points. Six animals were injected with a PFC emulsion that also contained a fluorophore. As a control against false-positive results, wild-type mice (n = 3) were injected with a PFC emulsion, and atherosclerotic mice were injected with a saline solution (n = 2). The animals were sacrificed after the last MR imaging examination, after which high-spatial-resolution ex vivo MR imaging and bright-field and immunofluorescent histologic examination were performed.
RESULTS: (19)F MR signal was detected in vivo in plaques in the aortic arch and its branches. The SNR was found to significantly increase up to day 6 (P < .001), and the SNR of all mice at this time point was 13.4 ± 3.3. The presence of PFC and plaque in the excised vessels was then confirmed both through ex vivo (19)F MR imaging and histologic examination, while no signal was detected in the control animals. Immunofluorescent histologic findings confirmed the presence of PFC in plaque macrophages.
CONCLUSION: (19)F MR imaging allows the noninvasive in vivo detection of inflammation in atherosclerotic plaques in a mouse model of atherosclerosis and opens up new avenues for both the early detection of vulnerable atherosclerosis and the elucidation of inflammation mechanisms in atherosclerosis.
Mots-clé
Animals, Disease Models, Animal, Fluorine/diagnostic use, Inflammation/complications, Inflammation/diagnosis, Magnetic Resonance Imaging/methods, Male, Mice, Mice, Inbred C57BL, Plaque, Atherosclerotic/complications, Plaque, Atherosclerotic/diagnosis
Pubmed
Web of science
Création de la notice
20/02/2015 11:27
Dernière modification de la notice
20/08/2019 14:06
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