Silk fibroin matrices for the controlled release of nerve growth factor (NGF)
Details
Serval ID
serval:BIB_275E09EE00F5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Silk fibroin matrices for the controlled release of nerve growth factor (NGF)
Journal
Biomaterials
ISSN
0142-9612 (Print)
Publication state
Published
Issued date
10/2007
Volume
28
Number
30
Pages
4449-60
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Nerve conduits (NC) for peripheral nerve repair should guide the sprouting axons and physically protect the axonal cone from any damage. The NC should also degrade after completion of its function to obviate the need of subsequent explanation and should optionally be suitable for controlled drug release of embedded growth factors to enhance nerve regeneration. Silk fibroin (SF) is a biocompatible and slowly biodegradable biomaterial with excellent mechanical properties that could meet the above stated requirements. SF material (films) supported the adherence and metabolic activity of PC12 cells, and, in combination with nerve growth factor (NGF), supported neurite outgrowth during PC12 cell differentiation. NGF-loaded SF-NC were prepared from aqueous solutions of NGF and SF (20%, w/w), which were air-dried or freeze-dried (freezing at -20 or -196 degrees C) in suitable molds. NGF release from the three differently prepared SF-NC was prolonged over at least 3 weeks, but the total amount released depended on the drying procedure of the NC. The potency of released NGF was retained within all formulations. Control experiments with differently dried NGF-lactose solutions did not evidence marked protein aggregation (SEC, HPLC), loss of ELISA-reactivity or PC12 cell bioactivity. This study encourages the further exploitation of SF-NC for growth factor delivery and evaluation in peripheral nerve repair.
Keywords
Acetates/chemistry
Animals
Area Under Curve
Biocompatible Materials/*chemistry
Bombyx/chemistry
Buffers
Calorimetry, Differential Scanning
Cattle
Cell Differentiation
Cell Proliferation
Chromatography, Gel
Chromatography, High Pressure Liquid
Collagen Type I/chemistry
Delayed-Action Preparations
Enzyme-Linked Immunosorbent Assay
Fibroins/*chemistry/isolation & purification/ultrastructure
Hydrogen-Ion Concentration
Laminin/chemistry
Nerve Growth Factor/analysis/immunology/*pharmacokinetics
Neurites/metabolism
PC12 Cells
Rats
Silk/*chemistry/isolation & purification/ultrastructure
Spectroscopy, Fourier Transform Infrared
Substrate Specificity
Pubmed
Web of science
Create date
28/01/2008 9:22
Last modification date
20/08/2019 14:06