Article: article from journal or magazin.
Neuronal traits are required for glucose-induced insulin secretion.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
The transcriptional repressor RE1 silencer transcription factor (REST) is an important factor that restricts some neuronal traits to neurons. Since these traits are also present in pancreatic beta-cells, we evaluated their role by generating a model of insulin-secreting cells that express REST. The presence of REST led to a decrease in expression of its known target genes, whereas insulin expression and its cellular content were conserved. As a consequence of REST expression, the capacity to secrete insulin in response to mitochondrial fuels, a particularity of mature beta-cells, was impaired. These data provide evidence that REST target genes are required for an appropriate glucose-induced insulin secretion.
Animals, Blotting, Northern, Cell Line, Coloring Agents, DNA, Complementary, DNA-Binding Proteins, Glucose, Insulin, Islets of Langerhans, Mice, Nerve Tissue Proteins, Neurons, Potassium Chloride, Promoter Regions, Genetic, RNA, RNA, Messenger, Repressor Proteins, Tetrazolium Salts, Thiazoles, Time Factors, Transcription, Genetic, Transfection
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