Molecular characterization of T-cell antigen receptor expression by subsets of CD4- CD8- murine thymocytes.

Détails

ID Serval
serval:BIB_262FA6B9186C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular characterization of T-cell antigen receptor expression by subsets of CD4- CD8- murine thymocytes.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Pearse M., Gallagher P., Wilson A., Wu L., Fisicaro N., Miller J.F., Scollay R., Shortman K.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1988
Volume
85
Numéro
16
Pages
6082-6086
Langue
anglais
Résumé
Precursors of all T-lineage cells are found in a population of thymocytes that lack the CD4 and CD8 surface glycoproteins. These "double-negative" thymocytes are markedly heterogeneous in their expression of other surface markers and include cells at various stages of development. In this study, CD4- CD8- adult murine thymocytes were separated into subsets based on the expression of the "heat stable antigen" (HSA) and of Ly 1 (CD5). The sorted subsets were analyzed directly (without prior expansion in culture) for T-cell antigen receptor (TcR) gene rearrangement and mRNA expression and for TcR and CD3 cell-surface protein expression. Very little surface CD3 or TcR expression was detected on the major HSA+ Ly 1low subset. However, the HSA+ Ly 1high, HSA- Ly 1high, and HSA- Ly 1low subsets all contained cells with surface expression of CD3 and TcR. In contrast to previous studies, we found no subset that exclusively expressed either the alpha beta or gamma delta heterodimer, although the ratio of alpha beta+ to gamma delta+ varied widely. Two of these three subsets (HSA- Ly 1low and HSA- Ly 1high) showed very high usage of V beta 8 gene products in the alpha beta heterodimer, but nevertheless included approximately equal to 15% non-V beta 8 alpha beta forms. All CD4- CD8- subsets were found to have extensively rearranged their TcR gamma genes and to express gamma mRNA. Expression of a high ratio of mature [1.3 kilobases (kb)] to truncated (1.0 kb) beta message and presence of alpha message was largely restricted to subsets with TcR alpha beta surface expression.
Mots-clé
Animals, Antigens, CD3, Antigens, Differentiation, T-Lymphocyte/analysis, Antigens, Surface/analysis, Male, Mice, Mice, Inbred CBA, RNA, Messenger/analysis, Receptors, Antigen, T-Cell/analysis, Receptors, Antigen, T-Cell/genetics, Recombination, Genetic, T-Lymphocytes/classification, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/04/2013 12:56
Dernière modification de la notice
20/08/2019 13:04
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