Response characteristics of arsenic-sensitive bioreporters expressing the gfp reporter gene

Details

Serval ID
serval:BIB_26268783A915
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Response characteristics of arsenic-sensitive bioreporters expressing the gfp reporter gene
Journal
Microchimica Acta
Author(s)
Wells M., Gösch M., Harms H., Van Der Meer J. R.
ISSN
0026-3672
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
151
Number
3-4
Pages
209-216
Language
english
Abstract
This paper describes the development of an analytical technique for arsenic analyses that is based on genetically-modified bioreporter bacteria bearing a gene encoding for the production of a green fluorescent protein (gfp). Upon exposure to arsenic (in the aqueous form of arsenite), the bioreporter production of the fluorescent reporter molecule is monitored spectroscopically. We compared the response measured as a function of time and concentration by steady-state fluorimetry (SSF) to that measured by epi-fluorescent microscopy (EFM). SSF is a bulk technique; as such it inherently yields less information, whereas EFM monitors the response of many individual cells simultaneously and data can be processed in terms of population averages or subpopulations. For the bioreporter strain used here, as well as for the literature we cite, the two techniques exhibit similar performance characteristics. The results presented here show that the EFM technique can compete with SSF and shows substantially more promise for future improvement; it is a matter of research interest to develop optimized methods of EFM image analysis and statistical data treatment. EFM is a conduit for understanding the dynamics of individual cell response vs. population response, which is not only a matter of research interest, but is also promising in the practical terms of developing micro-scale analysis.
Keywords
E. coli DH5 alpha, gfp, bioreporter, arsenic, epi-fluorescent microscopy, fluorimetry
Web of science
Create date
21/01/2008 13:35
Last modification date
20/08/2019 13:04
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