Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite.

Details

Serval ID
serval:BIB_253BCC1F0ECA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite.
Journal
Journal of Pharmaceutical and Biomedical Analysis
Author(s)
Déglon J., Thomas A., Daali Y., Lauer E., Samer C., Desmeules J., Dayer P., Mangin P., Staub C.
ISSN
1873-264X (Electronic)
ISSN-L
0731-7085
Publication state
Published
Issued date
2011
Volume
54
Number
2
Pages
359-367
Language
english
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article
Abstract
This paper illustrates the development of an automated system for the on-line bioanalysis of dried blood spots (on-line DBS). In this way, a prototype was designed for integration into a conventional LC/MS/MS, allowing the successive extraction of 30 DBS toward the analytical system without any sample pretreatment. The developed method was assessed for the DBS analysis of flurbiprofen (FLB) and its metabolite 4-hydroxyflurbiprofen (OH-FLB) in human whole blood (i.e. 5 μL). The automated procedure was fully validated based on international criteria and showed good precision, trueness, and linearity over the expected concentration range (from 10 to 1000 ng/mL and 100 to 10,000 ng/mL for OH-FLB and FLB respectively). Furthermore, the prototype showed good results in terms of recovery and carry-over. Stability of both analytes on filter paper was also investigated and the results suggested that DBS could be stored at ambient temperature for over 1 month. The on-line DBS automated system was then successfully applied to a pharmacokinetic study performed on healthy male volunteers after oral administration of a single 50-mg dose of FLB. Additionally, a comparison between finger capillary DBS and classic venous plasma concentrations was investigated. A good correlation was observed, demonstrating the complementarity of both sampling forms. The automated system described in this article represents an efficient tool for the LC/MS/MS analysis of DBS samples in many bioanalytical applications.
Keywords
Administration, Oral, Adsorption, Blood Chemical Analysis/methods, Calibration, Chromatography, Liquid/methods, Desiccation, Drug Stability, Drug Storage, Flurbiprofen/administration & dosage, Flurbiprofen/blood, Guidelines as Topic, Humans, Male, Micropore Filters, Online Systems, Quality Control, Reference Standards, Reproducibility of Results, Specimen Handling/methods, Tablets, Tandem Mass Spectrometry/methods
Pubmed
Web of science
Create date
22/03/2011 16:22
Last modification date
20/08/2019 13:03
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