Replication of a plasmid containing two origins of bacteriophage.

Details

Serval ID
serval:BIB_25110625D587
Type
Article: article from journal or magazin.
Collection
Publications
Title
Replication of a plasmid containing two origins of bacteriophage.
Journal
Journal of Molecular Biology
Author(s)
Dotto G.P., Horiuchi K.
ISSN
0022-2836 (Print)
ISSN-L
0022-2836
Publication state
Published
Issued date
1981
Volume
153
Number
1
Pages
169-176
Language
english
Abstract
A chimeric plasmid was constructed that contains a tandem duplication of the bacteriophage f1 origin of DNA replication. This plasmid replicates stably in the absence of phage. When cells carrying this plasmid are infected with f1, two new plasmid-derived DNA species are generated: a smaller, chimeric plasmid containing only one f1 origin of replication, and a miniphage, the genome of which consists of the f1 fragment that was located between the two f1 origins of the original plasmid. These data support the hypothesis (Horiuchi, 1980) that the nucleotide sequence recognized for initiation of plus strand synthesis in f1 DNA replication is also the signal for its termination.
Keywords
Chimera, Coliphages/genetics, DNA Replication, DNA, Viral, Electrophoresis, Agar Gel, Escherichia coli/genetics, Plasmids, Virus Replication
Pubmed
Web of science
Create date
24/01/2008 14:58
Last modification date
20/08/2019 13:03
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