PPAR-β/δ activation promotes phospholipid transfer protein expression.

Détails

ID Serval
serval:BIB_2432FF8C16ED
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
PPAR-β/δ activation promotes phospholipid transfer protein expression.
Périodique
Biochemical Pharmacology
Auteur(s)
Chehaibi K., Cedó L., Metso J., Palomer X., Santos D., Quesada H., Naceur Slimane M., Wahli W., Julve J., Vázquez-Carrera M., Jauhiainen M., Blanco-Vaca F., Escolà-Gil J.C.
ISSN
1873-2968 (Electronic)
ISSN-L
0006-2952
Statut éditorial
Publié
Date de publication
2015
Volume
94
Numéro
2
Pages
101-108
Langue
anglais
Résumé
The peroxisome proliferator-activated receptor (PPAR)-β/δ has emerged as a promising therapeutic target for treating dyslipidemia, including beneficial effects on HDL cholesterol (HDL-C). In the current study, we determined the effects of the PPAR-β/δ agonist GW0742 on HDL composition and the expression of liver HDL-related genes in mice and cultured human cells. The experiments were carried out in C57BL/6 wild-type, LDL receptor (LDLR)-deficient mice and PPAR-β/δ-deficient mice treated with GW0742 (10mg/kg/day) or a vehicle solution for 14 days. GW0742 upregulated liver phospholipid transfer protein (Pltp) gene expression and increased serum PLTP activity in mice. When given to wild-type mice, GW0742 significantly increased serum HDL-C and HDL phospholipids; GW0742 also raised serum potential to generate preβ-HDL formation. The GW0742-mediated effects on liver Pltp expression and serum enzyme activity were completely abolished in PPAR-β/δ-deficient mice. GW0742 also stimulated PLTP mRNA expression in mouse J774 macrophages, differentiated human THP-1 macrophages and human hepatoma Huh7. Collectively, our findings demonstrate a common transcriptional upregulation by GW0742-activated PPAR-β/δ of Pltp expression in cultured cells and in mouse liver resulting in enhanced serum PLTP activity. Our results also indicate that PPAR-β/δ activation may modulate PLTP-mediated preβ-HDL formation and macrophage cholesterol efflux.
Mots-clé
Animals, Cell Line, Humans, Mice, Mice, Inbred C57BL, PPAR delta/agonists, PPAR delta/metabolism, PPAR-beta/agonists, PPAR-beta/metabolism, Phospholipid Transfer Proteins/metabolism, Thiazoles/pharmacology
Pubmed
Web of science
Création de la notice
29/06/2015 11:08
Dernière modification de la notice
03/03/2018 14:57
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