Diurnal regulation of RNA polymerase III transcription is under the control of both the feeding-fasting response and the circadian clock.

Détails

Ressource 1Télécharger: Genome Res.-2017-Mange-973-84.pdf (1488.56 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_240C54C8CA8A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Diurnal regulation of RNA polymerase III transcription is under the control of both the feeding-fasting response and the circadian clock.
Périodique
Genome research
Auteur(s)
Mange F., Praz V., Migliavacca E., Willis I.M., Schütz F., Hernandez N.
Collaborateur(s)
CycliX Consortium
Contributeur(s)
Hernandez N., Delorenzi M., Deplancke B., Desvergne B., Guex N., Herr W., Naef F., Rougemont J., Schibler U., Andersin T., Cousin P., Gilardi F., Lammers F., Mange F., Villeneuve D., David F., Fabbretti R., Jacquet P., Krier I., Kuznetsov D., Leleu M., Liechti R., Martin O., Migliavacca E., Naldi A., Praz V., Rib L., Sobel J., Vlegel V., Xenarios I.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
06/2017
Peer-reviewed
Oui
Volume
27
Numéro
6
Pages
973-984
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
RNA polymerase III (Pol III) synthesizes short noncoding RNAs, many of which are essential for translation. Accordingly, Pol III activity is tightly regulated with cell growth and proliferation by factors such as MYC, RB1, TRP53, and MAF1. MAF1 is a repressor of Pol III transcription whose activity is controlled by phosphorylation; in particular, it is inactivated through phosphorylation by the TORC1 kinase complex, a sensor of nutrient availability. Pol III regulation is thus sensitive to environmental cues, yet a diurnal profile of Pol III transcription activity is so far lacking. Here, we first use gene expression arrays to measure mRNA accumulation during the diurnal cycle in the livers of (1) wild-type mice, (2) arrhythmic javax.xml.bind.JAXBElement@59c2c50e knockout mice, (3) mice fed at regular intervals during both night and day, and (4) mice lacking the javax.xml.bind.JAXBElement@160cb27a gene, and so provide a comprehensive view of the changes in cyclic mRNA accumulation occurring in these different systems. We then show that Pol III occupancy of its target genes rises before the onset of the night, stays high during the night, when mice normally ingest food and when translation is known to be increased, and decreases in daytime. Whereas higher Pol III occupancy during the night reflects a MAF1-dependent response to feeding, the rise of Pol III occupancy before the onset of the night reflects a circadian clock-dependent response. Thus, Pol III transcription during the diurnal cycle is regulated both in response to nutrients and by the circadian clock, which allows anticipatory Pol III transcription.

Mots-clé
ARNTL Transcription Factors/deficiency, ARNTL Transcription Factors/genetics, Animals, Circadian Clocks/genetics, Circadian Rhythm/genetics, Eating/genetics, Fasting/metabolism, Gene Expression Profiling, Gene Expression Regulation, Liver/metabolism, Mice, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Protein Binding, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, Repressor Proteins/deficiency, Repressor Proteins/genetics, Signal Transduction, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/04/2017 19:09
Dernière modification de la notice
20/08/2019 14:01
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