CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding.

Détails

Ressource 1Télécharger: BIB_23DA674EF09A.P001.pdf (211.02 [Ko])
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_23DA674EF09A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding.
Périodique
The Journal of experimental medicine
Auteur(s)
Renard V., Romero P., Vivier E., Malissen B., Luescher I.F.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
184
Numéro
6
Pages
2439-4244
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
To study the role of CD8 beta in T cell function, we derived a CD8 alpha/beta-(CD8-/-) T cell hybridoma of the H-2Kd-restricted N9 cytotoxic T lymphocyte clone specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260. This hybridoma was transfected either with CD8 alpha alone or together with CD8 beta. All three hybridomas released interleukin 2 upon incubation with L cells expressing Kd-peptide derivative complexes, though CD8 alpha/beta cells did so more efficiently than CD8 alpha/alpha and especially CD8-/- cells. More strikingly, only CD8 alpha/beta cells were able to recognize a weak agonist peptide derivative variant. This recognition was abolished by Fab' fragments of the anti-Kd alpha 3 monoclonal antibody SF1-1.1.1 or substitution of Kd D-227 with K, both conditions known to impair CD8 coreceptor function. T cell receptor (TCR) photoaffinity labeling indicated that TCR-ligand binding on CD8 alpha/beta cells was approximately 5- and 20-fold more avid than on CD8 alpha/a and CD8-/- cells, respectively. SF1-1.1.1 Fab' or Kd mutation D227K reduced the TCR photoaffinity labeling on CD8 alpha/beta cells to approximately the same low levels observed on CD8-/- cells. These results indicate that CD8 alpha/beta is a more efficient coreceptor than CD8alpha/alpha, because it more avidly strengthens TCR-ligand binding.
Mots-clé
Amino Acid Sequence, Animals, Antigens, CD8/immunology, Cell Line, Flow Cytometry, H-2 Antigens/immunology, Hybridomas/immunology, Mice, Peptide Fragments/immunology, Plasmodium berghei/immunology, Protozoan Proteins/immunology, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/immunology, Thymoma, Thymus Neoplasms
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:20
Dernière modification de la notice
08/05/2019 15:49
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