The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor.

Détails

ID Serval
serval:BIB_23828F6291A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor.
Périodique
Journal of Experimental Medicine
Auteur(s)
Gilliet M., Boonstra A., Paturel C., Antonenko S., Xu X.L., Trinchieri G., O'Garra A., Liu Y.J.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
2002
Volume
195
Numéro
7
Pages
953-958
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Plasmacytoid predendritic cells or type 1 interferon (IFN)-producing cells (IPCs) have recently been identified in mice. Although culture systems giving rise to different murine dendritic cell subsets have been established, the developmental regulation of murine plasmacytoid IPCs and the culture conditions leading to their generation remain unknown. Here we show that large numbers of over 40% pure CD11c(+)CD11b(-)B220(+)Gr-1(+) IPCs can be generated from mouse bone marrow cultures with FLT3-ligand. By contrast GM-CSF or TNF-alpha, which promote the generation of CD11c(+)CD11b(+)B220(-) myeloid DCs, block completely the development of IPCs. IPCs generated display similar features to human IPCs, such as the plasmacytoid morphology, the ability to produce large amounts of IFN-alpha in responses to herpes simplex virus, and the capacity to respond to ligands for Toll-like receptor 9 (TLR-9; CpG ODN 1668), but not to ligands for TLR-4 (lipopolysaccharide [LPS]). Unlike human IPCs which produce little IL-12p70, mouse IPCs produce IL-12p70 in response to CpG ODN 1668 and herpes simplex virus. This study demonstrates that the development of murine CD11c(+)CD11b(-)B220(+)Gr-1(+) IPCs and CD11c(+)CD11b(+)B220(-) myeloid DCs is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor. Human IPCs and mouse IPCs display different ability to produce IL-12p70. Large numbers of mouse IPCs can now be obtained from total bone marrow culture.
Mots-clé
Animals, Antigens, Differentiation/analysis, Bone Marrow Cells/cytology, Bone Marrow Cells/drug effects, Cell Differentiation, Cells, Cultured, Dendritic Cells/drug effects, Dendritic Cells/physiology, Female, Gene Expression Regulation, Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology, Hematopoiesis, Humans, Kinetics, Macrophage-1 Antigen/analysis, Membrane Proteins, Mice, Mice, Inbred BALB C, Species Specificity, Time Factors, Tumor Necrosis Factor-alpha/pharmacology
Pubmed
Web of science
Création de la notice
19/11/2012 19:31
Dernière modification de la notice
03/03/2018 14:54
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