Purified human plasma kallikrein aggregates human blood neutrophils

Details

Serval ID
serval:BIB_231EB8FFA57A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Purified human plasma kallikrein aggregates human blood neutrophils
Journal
Journal of Clinical Investigation
Author(s)
Schapira  M., Despland  E., Scott  C. F., Boxer  L. A., Colman  R. W.
ISSN
0021-9738 (Print)
Publication state
Published
Issued date
05/1982
Volume
69
Number
5
Pages
1199-202
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: May
Abstract
Exposure of human blood polymorphonuclear leukocytes (PMN) to purified active plasma kallikrein resulted in PMN aggregation when kallikrein was present at concentrations ranging from 0.4 to 0.6 U/ml (0.18-0.27 microM). Kallikrein-induced PMN aggregation was not mediated through C5-derived peptides, because identical responses were observed whether or not kallikrein had been preincubated with an antibody to C5. Moreover, kallikrein was specific for aggregating PMN, because no aggregation was observed with Factor XII active fragments (23 nM), Factor XIa (0.6 U/ml or 15nM), thrombin (1.6 microM), plasmin (2 microM), porcine pancreatic elastase (2 microM), bovine pancreatic chymotrypsin (2 microM), or bradykinin (1 microM). Bovine pancreatic trypsin (2 microM) aggregated PMN, but to a lesser extent than kallikrein (0.18 microM). Kallikrein was a potent aggregant agent for PMN because similar responses were observed with kallikrein (0.5 U/ml or 0.23 microM) and an optimal dose (0.2 microM) of N-formyl-methionyl-leucyl-phenylalanine. In addition, PMN incubation with kallikrein resulted in stimulation of their oxidative metabolism as assessed by an increased oxygen uptake. Neutropenia and leukostasis observed in diseases associated with activation of the contact phase system may be the result of PMN aggregation by plasma kallikrein.
Keywords
Cell Aggregation/drug effects Humans Kallikreins/blood/*pharmacology Neutrophils/*drug effects/metabolism Oxygen Consumption
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:28
Last modification date
20/08/2019 13:00
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