Article: article from journal or magazin.
Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma.
Clinical Cancer Research
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
The first tissue-specific angiogenic molecule, endocrine gland-derived vascular endothelial growth factor (EG-VEGF), was identified recently in human ovary, raising hopes of developing tumor type-specific angiogenesis inhibitors. In the present study, we analyzed the expression of EG-VEGF mRNA in normal human tissues and ovarian neoplasms by quantitative real-time reverse transcription-PCR. EG-VEGF mRNA was expressed in all ovarian neoplasms examined. No significant difference was identified among benign, low malignant potential neoplasms or stage I ovarian cancer, all of which exhibited 2-fold lower mRNA levels compared with normal premenopausal ovaries. EG-VEGF mRNA levels further decreased in late stage compared with early stage carcinomas (P < 0.05) and were consistently lower in laser capture microdissected tumor islets compared with surrounding stroma. EG-VEGF was undetectable by reverse transcription-PCR in 17 established epithelial ovarian cancer cell lines or in cultured human ovarian surface epithelial cells, whereas it was detected in peripheral blood as well as tumor-infiltrating T lymphocytes. Finally, in contrast to VEGF, EG-VEGF mRNA levels did not correlate with clinical outcome in advanced ovarian carcinoma. These results suggest that EG-VEGF is most likely derived from nonepithelial components of ovarian carcinomas and may play a marginal role in promoting angiogenesis in advanced ovarian carcinoma. We postulate that EG-VEGF-targeted antiangiogenic therapy may prove useful in early stage but not in advanced stage ovarian carcinoma.
Angiogenesis Inducing Agents/biosynthesis, Carcinoma/metabolism, Endocrine Glands/metabolism, Endothelial Growth Factors/biosynthesis, Female, Flow Cytometry, Gastrointestinal Hormones/biosynthesis, Humans, Immunohistochemistry, Intercellular Signaling Peptides and Proteins/biosynthesis, Lasers, Lymphokines/biosynthesis, Microscopy, Fluorescence, Ovarian Neoplasms/metabolism, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tissue Distribution, Treatment Outcome, Tumor Cells, Cultured, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor, Endocrine-Gland-Derived, Vascular Endothelial Growth Factors
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