Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins.

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Serval ID
serval:BIB_22EE60B2A2BD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins.
Journal
Science
Author(s)
Park S.Y., Fung P., Nishimura N., Jensen D.R., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow T.F., Alfred S.E., Bonetta D., Finkelstein R., Provart N.J., Desveaux D., Rodriguez P.L., McCourt P., Zhu J.K., Schroeder J.I., Volkman B.F., Cutler S.R.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Publication state
Published
Issued date
22/05/2009
Peer-reviewed
Oui
Volume
324
Number
5930
Pages
1068-1071
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
Type 2C protein phosphatases (PP2Cs) are vitally involved in abscisic acid (ABA) signaling. Here, we show that a synthetic growth inhibitor called pyrabactin functions as a selective ABA agonist. Pyrabactin acts through PYRABACTIN RESISTANCE 1 (PYR1), the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy.
Keywords
Abscisic Acid/agonists, Abscisic Acid/metabolism, Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis/growth & development, Arabidopsis/metabolism, Arabidopsis Proteins/antagonists & inhibitors, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Genes, Plant, Germination/drug effects, Ligands, Membrane Transport Proteins/genetics, Membrane Transport Proteins/metabolism, Mutation, Naphthalenes/chemistry, Naphthalenes/metabolism, Naphthalenes/pharmacology, Phosphoprotein Phosphatases/antagonists & inhibitors, Phosphoprotein Phosphatases/metabolism, Protein Binding, Recombinant Fusion Proteins/metabolism, Seeds/growth & development, Seeds/metabolism, Signal Transduction, Sulfonamides/chemistry, Sulfonamides/metabolism, Sulfonamides/pharmacology, Two-Hybrid System Techniques
DOI
10.1126/science.1173041
Pubmed
19407142
Web of science
000266246700041
Create date
10/01/2019 18:26
Last modification date
21/08/2019 6:35
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