Nasal microbiota composition dynamics after occupational change in animal farmers suggest major shifts.
Details
State: Public
Version: Author's accepted manuscript
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_22C37527CBE2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nasal microbiota composition dynamics after occupational change in animal farmers suggest major shifts.
Journal
The Science of the total environment
ISSN
1879-1026 (Electronic)
ISSN-L
0048-9697
Publication state
Published
Issued date
15/08/2021
Peer-reviewed
Oui
Volume
782
Pages
146842
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Previous studies have suggested a significantly higher diversity in the nasal microbiota of pig farmers compared to people having no contact with farm animals. However, the fate of this nasal microbiota specificity after farmers stop being in contact with the pig farm environment is unknown. The aim of this study was to investigate the change in the nasal microbiota of pig-farmers after the change of occupation. METHODS: Anterior and posterior nasal swabs were collected from seven people during employment on pig farms, and again after a period of at least 50 days after leaving the pig farm. Illumina MiSeq sequencing of 16S rRNA was conducted to characterize the dynamics of the nasal microbiota. The microbiota of actively working pig farmers was compared to microbiota after they had stopped working (ex-pig-farmers) and to control groups (cow farmers and non-exposed individuals). RESULTS: Following a prolonged period without exposure to pigs, α-diversity of both anterior and posterior cavities dropped significantly. The composition of the microbiota of pig-farmers had a low inter-similarity with the non-exposed group while ex-pig-farmers were more similar to cow-farmers and the non-exposed group than to their own microbiota during pig farming.
Keywords
Animals, Cattle, Farmers, Farms, Female, Humans, Microbiota, Nose, RNA, Ribosomal, 16S, Swine, Cow-farmers, Microbial diversity, Microbiome, Occupational health, Pig-farmer
Pubmed
Web of science
Create date
04/05/2021 8:08
Last modification date
25/05/2022 6:08