Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines
Details
Serval ID
serval:BIB_225DC56CF222
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines
Journal
Prostaglandins Leukot Essent Fatty Acids
ISSN
0952-3278 (Print)
ISSN-L
0952-3278
Publication state
Published
Issued date
10/1995
Peer-reviewed
Oui
Volume
53
Number
4
Pages
301-8
Language
english
Notes
Fontana, A
Di Marzo, V
Cadas, H
Piomelli, D
eng
Research Support, Non-U.S. Gov't
Scotland
Prostaglandins Leukot Essent Fatty Acids. 1995 Oct;53(4):301-8.
Di Marzo, V
Cadas, H
Piomelli, D
eng
Research Support, Non-U.S. Gov't
Scotland
Prostaglandins Leukot Essent Fatty Acids. 1995 Oct;53(4):301-8.
Abstract
Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquid-phase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.
Keywords
Animals, Arachidonic Acids/*chemistry/isolation & purification, Artifacts, Brain/cytology/metabolism, Cannabinoids/*chemistry/isolation & purification, Cells, Cultured, Chromatography, High Pressure Liquid, Chromatography, Liquid, Chromatography, Thin Layer, Endocannabinoids, Ethanolamines/chemical synthesis/*chemistry/isolation & purification, Gas Chromatography-Mass Spectrometry, Molecular Structure, Polyunsaturated Alkamides, Rats, Silica Gel, Silicon Dioxide
Pubmed
Create date
13/11/2017 10:57
Last modification date
20/08/2019 13:59
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