Rapid screening method for determination of Ecstasy and amphetamines in urine samples using gas chromatography-chemical ionisation mass spectrometry.

Détails

ID Serval
serval:BIB_22445
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rapid screening method for determination of Ecstasy and amphetamines in urine samples using gas chromatography-chemical ionisation mass spectrometry.
Périodique
Journal of Chromatography. B
Auteur(s)
Pellegrini M., Rosati F., Pacifici R., Zuccaro R., Romolo F.S., Lopez A.
ISSN
1570-0232
Statut éditorial
Publié
Date de publication
2002
Volume
769
Numéro
2
Pages
243-251
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The need for analytical screening tests more reliable and valid to detect amphetamine and related "designer drugs" in biological samples is becoming critical, due to the increasing diffusion of these drugs on the European illegal market. The most common screening procedures based on immunoassays suffer a number of limitations, including low sensitivity, lack of specificity and limited number of detectable substances. This paper describes a screening method based on gas-chromatography-mass-spectrometry (GC/MS) using positive chemical ionisation (PCI) detection. Methanol was used as reactant gas in the ionisation chamber. Molecular ions of different compounds were monitored, allowing a sensitivity of 5-10 ng/ml with high selectivity. The sensitivity of the method gives positive results in samples taken 48-72 h after intake of one dose of 50-100 mg. The method is simple and rapid. Sample preparation was limited to one liquid-liquid extraction, without any hydrolysis and derivatisation. Hydrolysis is critical to identify metabolites excreted as conjugates. Blank urine samples spiked with known amounts of amphetamine (AM), methylamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethylamphetamine (MDMA), methylenedioxyethylamphetamine (MDEA) and methylenedioxyphenyl-N-methyl-2-butanamine (MBDB) were analysed. The method was successfully tested on real samples of urine from people, whose use of amphetamine was suspected, and results were compared with results obtained with immunoassays.
Mots-clé
Adolescent, Adult, Amphetamines/urine, Female, Gas Chromatography-Mass Spectrometry/methods, Humans, Male, Middle Aged, N-Methyl-3,4-methylenedioxyamphetamine/urine, Sensitivity and Specificity
Pubmed
Création de la notice
19/11/2007 10:46
Dernière modification de la notice
03/03/2018 14:49
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