Article: article from journal or magazin.
Brain-derived neurotrophic factor enhances the expression of the monocarboxylate transporter 2 through translational activation in mouse cultured cortical neurons.
Journal of Cerebral Blood Flow and Metabolism
MCT2 is the predominant neuronal monocarboxylate transporter allowing lactate use as an alternative energy substrate. It is suggested that MCT2 is upregulated to meet enhanced energy demands after modifications in synaptic transmission. Brain-derived neurotrophic factor (BDNF), a promoter of synaptic plasticity, significantly increased MCT2 protein expression in cultured cortical neurons (as shown by immunocytochemistry and western blot) through a translational regulation at the synaptic level. Brain-derived neurotrophic factor can cause translational activation through different signaling pathways. Western blot analyses showed that p44/p42 mitogen-activated protein kinase (MAPK), Akt, and S6 were strongly phosphorylated on BDNF treatment. To determine by which signal transduction pathway(s) BDNF mediates its upregulation of MCT2 protein expression, the effect of specific inhibitors for p38 MAPK, phosphoinositide 3-kinase (PI3K), mammalian target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK), p44/p42 MAPK (ERK), and Janus kinase 2 (JAK2) was evaluated. It could be observed that the BDNF-induced increase in MCT2 protein expression was almost completely blocked by all inhibitors, except for JAK2. These data indicate that BDNF induces an increase in neuronal MCT2 protein expression by a mechanism involving a concomitant stimulation of PI3K/Akt/mTOR/S6, p38 MAPK, and p44/p42 MAPK. Moreover, our observations suggest that changes in MCT2 expression could participate in the process of synaptic plasticity induced by BDNF.
Animals, Blotting, Western, Brain-Derived Neurotrophic Factor/metabolism, Cells, Cultured, Cerebral Cortex/drug effects, Cerebral Cortex/metabolism, Enzyme Inhibitors/pharmacology, Gene Expression, Gene Expression Regulation, Immunohistochemistry, Mice, Monocarboxylic Acid Transporters/biosynthesis, Monocarboxylic Acid Transporters/genetics, Neuronal Plasticity/drug effects, Neuronal Plasticity/physiology, Neurons/drug effects, Neurons/metabolism, Protein Biosynthesis/drug effects, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction/drug effects, Signal Transduction/physiology
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