Fas ligand expression in glioblastoma cell lines and primary astrocytic brain tumors.

Details

Serval ID
serval:BIB_2210
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fas ligand expression in glioblastoma cell lines and primary astrocytic brain tumors.
Journal
Brain Pathology
Author(s)
Gratas C., Tohma Y., Van Meir E.G., Klein M., Tenan M., Ishii N., Tachibana O., Kleihues P., Ohgaki H.
ISSN
1015-6305
Publication state
Published
Issued date
1997
Volume
7
Number
3
Pages
863-869
Language
english
Notes
Publication types: Clinical Trial
Abstract
Fas/APO-1 (CD95) is a cell surface receptor that mediates apoptosis when it reacts with Fas ligand (FasL) or Fas antibody. We previously reported that Fas expression is predominantly induced in perinecrotic glioma cells, suggesting that Fas induction is associated with apoptosis and necrosis formation, a histological hallmark of glioblastomas. In this study, we assessed the expression of FasL in 10 glioblastoma cell lines and in 14 astrocytic brain tumors (three low-grade astrocytomas and 11 glioblastomas). Reverse transcriptase (RT)-PCR revealed that all glioblastoma cell lines and primary astrocytic brain tumors express FasL. Immunohistochemically, FasL was predominantly expressed on the plasma membrane of glioma cells. These results suggest that FasL expression is common in human astrocytic brain tumors and may cause apoptosis of glioma cells if Fas expression is induced.
Keywords
Adult, Aged, Antigens, CD95/biosynthesis, Antigens, Neoplasm/biosynthesis, Apoptosis, Astrocytoma/immunology, Astrocytoma/pathology, Brain Neoplasms/immunology, Brain Neoplasms/pathology, Child, Fas Ligand Protein, Female, Glioblastoma/immunology, Glioblastoma/pathology, Humans, Ligands, Male, Membrane Glycoproteins/biosynthesis, Middle Aged, Polymerase Chain Reaction/methods, Transcription, Genetic, Tumor Cells, Cultured
Pubmed
Web of science
Create date
19/11/2007 12:17
Last modification date
20/08/2019 12:58
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