Monitoring time-dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry.

Détails

ID Serval
serval:BIB_21C3D60731EF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Monitoring time-dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry.
Périodique
Journal of Mass Spectrometry
Auteur(s)
Patterson NH, Thomas A., Chaurand P.
ISSN
1076-5174
ISSN-L
1076-5174
Statut éditorial
Publié
Date de publication
2014
Volume
49
Numéro
7
Pages
622-627
Langue
anglais
Résumé
Imaging mass spectrometry (IMS) is useful for visualizing the localization of phospholipids on biological tissue surfaces creating great opportunities for IMS in lipidomic investigations. With advancements in IMS of lipids, there is a demand for large-scale tissue studies necessitating stable, efficient and well-defined sample handling procedures. Our work within this article shows the effects of different storage conditions on the phospholipid composition of sectioned tissues from mouse organs. We have taken serial sections from mouse brain, kidney and liver thaw mounted unto ITO-coated glass slides and stored them under various conditions later analyzing them at fixed time points. A global decrease in phospholipid signal intensity is shown to occur and to be a function of time and temperature. Contrary to the global decrease, oxidized phospholipid and lysophospholipid species are found to increase within 2 h and 24 h, respectively, when mounted sections are kept at ambient room conditions. Imaging experiments reveal that degradation products increase globally across the tissue. Degradation is shown to be inhibited by cold temperatures, with sample integrity maintained up to a week after storage in −80 °C freezer under N2 atmosphere. Overall, the results demonstrate a timeline of the effects of lipid degradation specific to sectioned tissues and provide several lipid species which can serve as markers of degradation. Importantly, the timeline demonstrates oxidative sample degradation begins appearing within the normal timescale of IMS sample preparation of lipids (i.e. 1-2 h) and that long-term degradation is global. Taken together, these results strengthen the notion that standardized procedures are required for phospholipid IMS of large sample sets, or in studies where many serial sections are prepared together but analyzed over time such as in 3-D IMS reconstruction experiments.
Mots-clé
Forensic Medicine, MALDI, mass spectrometry, imaging, phospholipid, degradation
Pubmed
Web of science
Création de la notice
07/07/2014 15:26
Dernière modification de la notice
20/08/2019 12:58
Données d'usage