Article: article from journal or magazin.
Specific high-affinity binding of 125I-labeled mouse interferon to interfevon resistant embryonal carcinoma cells in vitro.
Multipotential mouse embryonal carcinoma cells are resistant to several biologic effects of mouse interferon: inhibition of viral multiplication and inhibition of cell division. Nevertheless using 125I-labeled highly purified mouse interferon we have shown that these embryonal carcinoma cells express specific interferon receptors in similar number and of the same affinity as interferon sensitive differentiated embryonal carcinoma cells. Thus, mechanisms of interferon resistance are probably multiple: some cells are resistant because they lack specific binding sites for interferon (interferon resistant mouse L1210 cells) and other cells, as shown herein, are resistant to some of the effects of interferon despite binding of interferon to specific receptor sites. Furthermore, these binding sites may be considered functional, since interferon does induce an increase in 2-5A synthetase in these cells.
Animals, Cell Differentiation, Cell Division/drug effects, Cell Line, Drug Resistance, Encephalomyocarditis virus/growth & development, Interferons/metabolism, Mice, Receptors, Cell Surface/metabolism, Receptors, Interferon, Teratoma, Vesicular stomatitis Indiana virus/growth & development, Virus Replication/drug effects
Web of science
Last modification date