T cell receptor specificity is critical for the development of epidermal gammadelta T cells.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_20717
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
T cell receptor specificity is critical for the development of epidermal gammadelta T cells.
Périodique
The Journal of Experimental Medicine
Auteur(s)
Ferrero I., Wilson A., Beermann F., Held W., MacDonald H.R.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
194
Numéro
10
Pages
1473-1483
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
A particular feature of gammadelta T cell biology is that cells expressing T cell receptor (TCR) using specific Vgamma/Vdelta segments are localized in distinct epithelial sites, e.g., in mouse epidermis nearly all gammadelta T cells express Vgamma3/Vdelta1. These cells, referred to as dendritic epidermal T cells (DETC) originate from fetal Vgamma3+ thymocytes. The role of gammadelta TCR specificity in DETC's migration/localization to the skin has remained controversial. To address this issue we have generated transgenic (Tg) mice expressing a TCR delta chain (Vdelta6.3-Ddelta1-Ddelta2-Jdelta1-Cdelta), which can pair with Vgamma3 in fetal thymocytes but is not normally expressed by DETC. In wild-type (wt) Vdelta6.3Tg mice DETC were present and virtually all of them express Vdelta6.3. However, DETC were absent in TCR-delta(-/-) Vdelta6.3Tg mice, despite the fact that Vdelta6.3Tg gammadelta T cells were present in normal numbers in other lymphoid and nonlymphoid tissues. In wt Vdelta6.3Tg mice, a high proportion of in-frame Vdelta1 transcripts were found in DETC, suggesting that the expression of an endogenous TCR-delta (most probably Vdelta1) was required for the development of Vdelta6.3+ epidermal gammadelta T cells. Collectively our data demonstrate that TCR specificity is essential for the development of gammadelta T cells in the epidermis. Moreover, they show that the TCR-delta locus is not allelically excluded.
Mots-clé
Animals, Cell Movement, Dendritic Cells/physiology, Mice, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, Antigen, T-Cell, gamma-delta/physiology, Skin/immunology, Stem Cells/physiology, T-Lymphocytes/physiology, Thymus Gland/cytology
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 12:15
Dernière modification de la notice
20/08/2019 12:56
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