Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling.

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Serval ID
serval:BIB_202F278D6524
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling.
Journal
Nucleic Acids Research
Author(s)
Benedetti F., Japaridze A., Dorier J., Racko D., Kwapich R., Burnier Y., Dietler G., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
2015
Volume
43
Number
4
Pages
2390-2399
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated from bacteria. What are then the specific advantages of reduced supercoiling density that is maintained in vivo? Using Brownian dynamics simulations and atomic force microscopy we show here that thanks to physiological DNA-DNA crowding DNA molecules with reduced supercoiling density are still sufficiently supercoiled to stimulate interaction between cis-regulatory elements. On the other hand, weak supercoiling permits DNA molecules to modulate their overall shape in response to physiological changes in DNA crowding. This plasticity of DNA shapes may have regulatory role and be important for the postreplicative spontaneous segregation of bacterial chromosomes.
Pubmed
Web of science
Open Access
Yes
Create date
24/04/2015 7:12
Last modification date
20/08/2019 12:56
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