Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling.

Détails

Ressource 1Télécharger: BIB_202F278D6524.P001.pdf (7562.35 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_202F278D6524
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling.
Périodique
Nucleic Acids Research
Auteur(s)
Benedetti F., Japaridze A., Dorier J., Racko D., Kwapich R., Burnier Y., Dietler G., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2015
Volume
43
Numéro
4
Pages
2390-2399
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated from bacteria. What are then the specific advantages of reduced supercoiling density that is maintained in vivo? Using Brownian dynamics simulations and atomic force microscopy we show here that thanks to physiological DNA-DNA crowding DNA molecules with reduced supercoiling density are still sufficiently supercoiled to stimulate interaction between cis-regulatory elements. On the other hand, weak supercoiling permits DNA molecules to modulate their overall shape in response to physiological changes in DNA crowding. This plasticity of DNA shapes may have regulatory role and be important for the postreplicative spontaneous segregation of bacterial chromosomes.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/04/2015 7:12
Dernière modification de la notice
20/08/2019 12:56
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