Role of phosphoinositide 3-kinase in the autophagic death of serum-deprived PC12 cells.

Détails

ID Serval
serval:BIB_1EF1B6863FBF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of phosphoinositide 3-kinase in the autophagic death of serum-deprived PC12 cells.
Périodique
Apoptosis : an International Journal on Programmed Cell Death
Auteur(s)
Guillon-Munos A., van Bemmelen M.X., Clarke P.G.
ISSN
1360-8185 (Print)
ISSN-L
1360-8185
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
10
Numéro
5
Pages
1031-1041
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The death of serum-deprived undifferentiated PC12 cells shows both autophagic and apoptotic features. Since it is still controversial whether the autophagy is instrumental in the cell death or a mere epiphenomenon, we tested the effects of inhibiting the autophagy by a variety of phosphoinositide 3-kinase inhibitors, and provided evidence that the autophagy, or a related trafficking event, is indeed instrumental in the cell death. Furthermore, by comparing the effects of PI3-K inhibition and caspase-inhibition on autophagic and apoptotic cellular events, we showed that in this case the autophagic and apoptotic mechanisms mediate cell death by parallel pathways and do not act in series.
Mots-clé
Acid Phosphatase/analysis, Animals, Apoptosis/drug effects, Apoptosis/physiology, Autophagy/drug effects, Autophagy/physiology, Caspase Inhibitors, Chromones/pharmacology, Culture Media, Serum-Free/pharmacology, Immunohistochemistry, Lysosomes/physiology, Microscopy, Electron, Morpholines/pharmacology, PC12 Cells/cytology, Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Phosphatidylinositol 3-Kinases/biosynthesis, Rats
Pubmed
Web of science
Création de la notice
20/01/2008 18:49
Dernière modification de la notice
03/03/2018 14:36
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