The response of Mucor plumbeus to pentachlorophenol: a toxicoproteomics study.

Details

Serval ID
serval:BIB_1ECE5482ADE4
Type
Article: article from journal or magazin.
Collection
Publications
Title
The response of Mucor plumbeus to pentachlorophenol: a toxicoproteomics study.
Journal
Journal of proteomics
Author(s)
Carvalho M.B., Martins I., Medeiros J., Tavares S., Planchon S., Renaut J., Núñez O., Gallart-Ayala H., Galceran M.T., Hursthouse A., Silva Pereira C.
ISSN
1876-7737 (Electronic)
ISSN-L
1874-3919
Publication state
Published
Issued date
14/01/2013
Volume
78
Pages
159-171
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Pentachlorophenol (PCP) represents a critical concern worldwide due to its toxicity and recalcitrance to degradation. The capacity of Mucor plumbeus to transform PCP into several detoxification metabolites, including tetrachlorohydroquinone and several phase II conjugates, was observed by LC-HRMS. The data obtained support the degradation pathway proposed previously. PCP effects in M. plumbeus, an unsequenced species, were investigated using a proteomics approach (bidimensional gel electrophoresis followed by MALDI TOF/TOF analyses). The mycelial proteins identified in the differentially accumulated spots allowed the identification of PCP responsive proteins. The presence of PCP increased the energy demand, altered the cell wall architecture and cytoskeleton and induced a significant stress response. The latter was emphasised by the up-accumulation of protein species associated with defence mechanisms (e.g. HSP70 and cytochrome c peroxidase). Overall the data produced corroborate the capability of PCP to uncouple oxidative-phosphorylation in mitochondria. Importantly, one of the identified mycelial protein species, a NAD- and Zn-dependent ADH, is likely to be involved in PCP degradation. Amongst the fungal secretome analysed, no putative PCP degradative enzymes were detected. This work constitutes the first toxicoproteomic study involving a Zygomycota fungus and the very first concerning the effect of PCP in a fungal proteome.

Keywords
Biotransformation/drug effects, Biotransformation/physiology, Environmental Pollutants/metabolism, Environmental Pollutants/pharmacology, Fungal Proteins/secretion, Mucor/metabolism, Pentachlorophenol/metabolism, Pentachlorophenol/pharmacology, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pubmed
Create date
19/04/2017 17:12
Last modification date
21/08/2019 5:34
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