Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake.

Détails

ID Serval
serval:BIB_1EB1BA42FA1D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake.
Périodique
Molecular and Cellular Proteomics
Auteur(s)
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
ISSN
1535-9476 (Print)
ISSN-L
1535-9476
Statut éditorial
Publié
Date de publication
2006
Volume
5
Numéro
12
Pages
2228-2243
Langue
anglais
Résumé
Phagocytosis, whether of food particles in protozoa or bacteria and cell remnants in the metazoan immune system, is a conserved process. The particles are taken up into phagosomes, which then undergo complex remodeling of their components, called maturation. By using two-dimensional gel electrophoresis and mass spectrometry combined with genomic data, we identified 179 phagosomal proteins in the amoeba Dictyostelium, including components of signal transduction, membrane traffic, and the cytoskeleton. By carrying out this proteomics analysis over the course of maturation, we obtained time profiles for 1,388 spots and thus generated a dynamic record of phagosomal protein composition. Clustering of the time profiles revealed five clusters and 24 functional groups that were mapped onto a flow chart of maturation. Two heterotrimeric G protein subunits, Galpha4 and Gbeta, appeared at the earliest times. We showed that mutations in the genes encoding these two proteins produce a phagocytic uptake defect in Dictyostelium. This analysis of phagosome protein dynamics provides a reference point for future genetic and functional investigations.
Mots-clé
Animals, Animals, Genetically Modified, Cells, Cultured, Cluster Analysis, Dictyostelium, Electrophoresis, Gel, Two-Dimensional, GTP-Binding Protein alpha Subunits/physiology, GTP-Binding Protein beta Subunits/genetics, Models, Biological, Phagocytosis, Phagosomes/metabolism, Proteomics/methods
Pubmed
Web of science
Création de la notice
22/04/2013 9:16
Dernière modification de la notice
03/03/2018 14:36
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