Fas ligand expression is restricted to nonlymphoid thymic components in situ.

Détails

ID Serval
serval:BIB_1EA9AE290E0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fas ligand expression is restricted to nonlymphoid thymic components in situ.
Périodique
Journal of immunology
Auteur(s)
French L.E., Wilson A., Hahne M., Viard I., Tschopp J., MacDonald H.R.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
159
Numéro
5
Pages
2196-2202
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The cell surface receptor Fas (Apo-1/CD95) and its ligand (FasL) are mediators of apoptosis that have been shown to be implicated in activation-induced death of mature T cells and in killing mediated by cytolytic T cells. The role of the Fas pathway in apoptosis associated with thymic selection events is, however, controversial. Although Fas and FasL are known to be expressed in the thymus, the nature and in vivo localization of FasL-expressing cells have not been determined. Using recently developed anti-FasL Abs in combination with in situ hybridization on tissue sections, we show in this work that FasL-expressing cells are present in the thymus, particularly within the medulla. FasL mRNA was detected readily in thymic stromal cell extracts, but not in isolated thymocytes. Moreover, immunohistochemical analysis of serial tissue sections stained with Abs against FasL in conjunction with epithelial and dendritic cell markers indicated that both thymic epithelial and dendritic cells express FasL in situ. The coexistence of FasL-expressing stromal cells and Fas-expressing thymocytes may have important implications for the role of the Fas pathway in apoptosis associated with thymic selection events.
Mots-clé
Animals, Antigens, CD95/physiology, Apoptosis/physiology, Cell Differentiation, Clonal Deletion/physiology, Connective Tissue/metabolism, Fas Ligand Protein, Gene Expression Regulation, In Situ Hybridization, Membrane Glycoproteins/biosynthesis, Membrane Glycoproteins/genetics, Mice, Mice, Inbred Strains, Organ Specificity, RNA, Messenger/analysis, Thymus Gland/cytology, Thymus Gland/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:19
Dernière modification de la notice
20/08/2019 12:54
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