CD3+ WT31- peripheral T lymphocytes lack T44 (CD28), a surface molecule involved in activation of T cells bearing the alpha/beta heterodimer

Détails

ID Serval
serval:BIB_1E827E82B055
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CD3+ WT31- peripheral T lymphocytes lack T44 (CD28), a surface molecule involved in activation of T cells bearing the alpha/beta heterodimer
Périodique
European Journal of Immunology
Auteur(s)
Poggi  A., Bottino  C., Zocchi  M. R., Pantaleo  G., Ciccone  E., Mingari  C., Moretta  L., Moretta  A.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
07/1987
Volume
17
Numéro
7
Pages
1065-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
We have applied two-color fluorescence cytofluorometric techniques to the analysis of the distribution of T44 and CD3 antigens in peripheral blood human lymphocytes. While most CD3+ cells co-expressed T44 antigen, a small distinct subset was CD3+ T44- (2-10% of CD3+ cells). This cell subset also did not react with the WT31 monoclonal antibody (mAb), specific for an alpha/beta framework determinant of the T cell receptor (TCR). Lack of T44 antigen expression was also observed in purified CD3+ WT31- polyclonal populations that had been cultured in medium containing interleukin 2 (IL2) and as well as greater than 30 clones expressing the CD3+4-8-WT31- surface phenotype. Immunoprecipitation experiments confirmed that expression of T44 molecules was confined to CD3+ WT31+ peripheral blood T cells. While conventional CD3+ WT31+ cells produced IL2 in response to mAb directed to CD2, CD3 or T44 surface molecules, CD3+ WT31- cells did not respond to anti-T44 mAb but released IL2 following stimulation with anti-CD2 or anti-CD3 mAb. Therefore, assuming that anti-T44 mimicks the effect of a still undefined natural ligand our data suggest that T cells expressing the gamma-gene surface product may be signalled by stimuli which differ, at least in part, from those acting on CD3+ WT31+ T lymphocytes.
Mots-clé
Antigens, Differentiation, T-Lymphocyte Antigens, Surface/analysis/*immunology Cells, Cultured Electrophoresis, Polyacrylamide Gel Epitopes/*analysis Flow Cytometry/methods Growth Humans Interleukin-2/metabolism *Lymphocyte Activation Phenotype Precipitin Tests Protein Conformation Receptors, Antigen, T-Cell/immunology Sodium Dodecyl Sulfate T-Lymphocytes/cytology/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 15:13
Dernière modification de la notice
20/08/2019 12:54
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