Activation of the heat shock response in plants by chlorophenols: transgenic Physcomitrella patens as a sensitive biosensor for organic pollutants.

Details

Serval ID
serval:BIB_1E5D38C5C137
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Activation of the heat shock response in plants by chlorophenols: transgenic Physcomitrella patens as a sensitive biosensor for organic pollutants.
Journal
Plant, Cell and Environment
Author(s)
Saidi Y., Domini M., Choy F., Zryd J.P., Schwitzguebel J.P., Goloubinoff P.
ISSN
0140-7791[print], 0140-7791[linking]
Publication state
Published
Issued date
2007
Volume
30
Number
6
Pages
753-763
Language
english
Abstract
The ability to detect early molecular responses to various chemicals is central to the understanding of biological impact of pollutants in a context of varying environmental cues. To monitor stress responses in a model plant, we used transgenic moss Physcomitrella patens expressing the beta-glucuronidase reporter (GUS) under the control of the stress-inducible promoter hsp17.3B. Following exposure to pollutants from the dye and paper industry, GUS activity was measured by monitoring a fluorescent product. Chlorophenols, heavy metals and sulphonated anthraquinones were found to specifically activate the hsp17.3B promoter (within hours) in correlation with long-term toxicity effects (within days). At mildly elevated physiological temperatures, the chemical activation of this promoter was strongly amplified, which considerably increased the sensitivity of the bioassay. Together with the activation of hsp17.3B promoter, chlorophenols induced endogenous chaperones that transiently protected a recombinant thermolabile luciferase (LUC) from severe heat denaturation. This sensitive bioassay provides an early warning molecular sensor to industrial pollutants under varying environments, in anticipation to long-term toxic effects in plants. Because of the strong cross-talk between abiotic and chemical stresses that we find, this P. patens line is more likely to serve as a direct toxicity bioassay for pollutants combined with environmental cues, than as an indicator of absolute toxicity thresholds for various pollutants. It is also a powerful tool to study the role of heat shock proteins (HSPs) in plants exposed to combined chemical and environmental stresses.
Keywords
Biosensing Techniques, Blotting, Western, Bryopsida/physiology, Chlorophenols/analysis, Chlorophenols/toxicity, Environmental Pollutants/analysis, Environmental Pollutants/toxicity, Heat-Shock Response, Plants, Genetically Modified/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 20:02
Last modification date
20/08/2019 12:54
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