In situ localization with digoxigenin-labelled probes of tau-related mRNAs in the rat pancreas.

Details

Serval ID
serval:BIB_1E3B84285188
Type
Article: article from journal or magazin.
Collection
Publications
Title
In situ localization with digoxigenin-labelled probes of tau-related mRNAs in the rat pancreas.
Journal
Histochemical Journal
Author(s)
Neuville P., Vanier M.T., Michalik L., Launay J.F.
ISSN
0018-2214[print], 0018-2214[linking]
Publication state
Published
Issued date
08/1995
Volume
27
Number
8
Pages
565-574
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Two cDNA probes complementary to fetal rat brain tau cDNA were produced by the polymerase chain reaction (PCR) and labelled by digoxigenin-11-dUTP incorporation during the PCR elongation step. These probes were tested for the in situ localization of tau mRNAs in sections of rat cerebellum. The hybridization signal was consistent with the known localization of brain tau mRNAs, showing the validity of cDNA probes labelled by digoxigenin during the PCR. Using these probes, an in situ hybridization protocol was established and optimized for the localization of tau-related mRNAs in sections of pancreas. The aim was to determine whether these mRNAs were expressed in the exocrine or the endocrine part of the pancreas. A positive signal was found only in the exocrine part of the pancreas, and was distributed exclusively in the cytoplasm of acinar cells. The results described here are the first evidence for a specific expression of tau-related proteins in the exocrine pancreas.
Keywords
Animals, Cerebellum/chemistry, Chromatography, Affinity, DNA Probes/analysis, Deoxyuracil Nucleotides, Digoxigenin/analogs &amp, derivatives, In Situ Hybridization, Indicators and Reagents, Microtomy, Pancreas/chemistry, Polymerase Chain Reaction, RNA, Messenger/analysis, RNA, Messenger/genetics, Rats, Reproducibility of Results, tau Proteins/analysis, tau Proteins/genetics
Pubmed
Web of science
Create date
24/01/2008 15:44
Last modification date
20/08/2019 12:54
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