Cholinergic neurons of fetal rat telencephalon in aggregating cell culture respond to NGF as well as to protein kinase C-activating tumor promoters.

Détails

ID Serval
serval:BIB_1E2A6F158EEC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cholinergic neurons of fetal rat telencephalon in aggregating cell culture respond to NGF as well as to protein kinase C-activating tumor promoters.
Périodique
Developmental Brain Research
Auteur(s)
Honegger P., Du Pasquier P., Tenot M.
ISSN
0165-3806
ISSN-L
0006-8993
Statut éditorial
Publié
Date de publication
1986
Volume
394
Numéro
2
Pages
217-223
Langue
anglais
Résumé
Serum-free aggregating cell cultures of fetal rat telencephalon treated with the potent tumor promoter phorbol 12-myristate 13-acetate (PMA) showed a dose-dependent, persistent stimulation of the enzymes choline acetyltransferase (ChAT), glutamic acid decarboxylase and glutamine synthetase. After elimination of the proliferating cells by treatment of the cultures with Ara-C (0.4 microM) only the cholinergic marker enzyme, ChAT, could be stimulated by tumor promoters. The non-promoting phorbol ester, 4 alpha-phorbol 12,13-didecanoate proved to be inactive in these cultures, whereas the potent non-phorbol tumor promoter, mezerein, produced an even greater stimulatory effect than PMA. Since PMA and mezerein are potent and specific activators of protein kinase C, the present results suggest a role for this second messenger in the development of cholinergic telencephalon neurons. Stimulation of ChAT required prolonged exposure (48 h) of the cultures to PMA and the responsiveness of the cholinergic neurons to the tumor promoters decreased with progressive cellular maturation. The cholinergic telencephalon neurons showed the same pattern of responsiveness for tumor promoters as for nerve growth factor (NGF). However, the combined treatment with NGF and either PMA or mezerein produced an additive stimulatory effect, suggesting somewhat different mechanisms of action.
Mots-clé
Animals, Cells, Cultured, Cholinergic Fibers/drug effects, Diterpenes, Nerve Growth Factors/pharmacology, Rats, Rats, Inbred Strains, Stimulation, Chemical, Telencephalon/drug effects, Telencephalon/embryology, Terpenes/pharmacology, Tetradecanoylphorbol Acetate/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
03/03/2018 14:35
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