Microscopy of Fission Yeast Sexual Lifecycle.

Détails

ID Serval
serval:BIB_1DE423310FC6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Microscopy of Fission Yeast Sexual Lifecycle.
Périodique
Journal of Visualized Experiments
Auteur(s)
Vjestica A., Merlini L., Dudin O., Bendezu F.O., Martin S.G.
ISSN
1940-087X (Electronic)
ISSN-L
1940-087X
Statut éditorial
Publié
Date de publication
2016
Numéro
109
Pages
e53801
Langue
anglais
Résumé
The fission yeast Schizosaccharomyces pombe has been an invaluable model system in studying the regulation of the mitotic cell cycle progression, the mechanics of cell division and cell polarity. Furthermore, classical experiments on its sexual reproduction have yielded results pivotal to current understanding of DNA recombination and meiosis. More recent analysis of fission yeast mating has raised interesting questions on extrinsic stimuli response mechanisms, polarized cell growth and cell-cell fusion. To study these topics in detail we have developed a simple protocol for microscopy of the entire sexual lifecycle. The method described here is easily adjusted to study specific mating stages. Briefly, after being grown to exponential phase in a nitrogen-rich medium, cell cultures are shifted to a nitrogen-deprived medium for periods of time suited to the stage of the sexual lifecycle that will be explored. Cells are then mounted on custom, easily built agarose pad chambers for imaging. This approach allows cells to be monitored from the onset of mating to the final formation of spores.
Mots-clé
Molecular Biology, Issue 109, Fission yeast, Schizosaccharomyces pombe, live-cell imaging, fluorescence microscopy, nitrogen starvation, pheromone, G protein-coupled receptor, sex, mating, shmoo, cell-cell fusion
Pubmed
Web of science
Création de la notice
09/04/2016 16:09
Dernière modification de la notice
03/03/2018 14:35
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