Article: article from journal or magazin.
The tfdK gene product facilitates uptake of 2,4-dichlorophenoxyacetate by Ralstonia eutropha JMP134(pJP4).
Journal of Bacteriology
Uptake of 2,4-dichlorophenoxyacetate (2,4-D) by Ralstonia eutropha JMP134(pJP4) was studied and shown to be an energy-dependent process. The uptake system was inducible with 2,4-D and followed saturation kinetics in a concentration range of up to 60 microM, implying the involvement of a protein in the transport process. We identified an open reading frame on plasmid pJP4, which was designated tfdK, whose translation product TfdK was highly hydrophobic and showed resemblance to transport proteins of the major facilitator superfamily. An interruption of the tfdK gene on plasmid pJP4 decimated 2,4-D uptake rates, which implies a role for TfdK in uptake. A tfdA mutant, which was blocked in the first step of 2,4-D metabolism, still took up 2,4-D. A mathematical model describing TfdK as an active transporter at low micromolar concentrations fitted the observed uptake data best.
2,4-Dichlorophenoxyacetic Acid/metabolism, Alcaligenes/genetics, Alcaligenes/metabolism, Amino Acid Sequence, Bacterial Proteins, Base Sequence, Biological Transport, Carrier Proteins/chemistry, Carrier Proteins/genetics, Escherichia coli/genetics, Escherichia coli/metabolism, Kinetics, Membrane Transport Proteins, Molecular Sequence Data, Plasmids, Sequence Alignment, Sequence Homology, Amino Acid
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