Determination of collagen fibril size via absolute measurements of second-harmonic generation signals.

Détails

ID Serval
serval:BIB_1DAB610021CF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of collagen fibril size via absolute measurements of second-harmonic generation signals.
Périodique
Nature Communications
Auteur(s)
Bancelin S., Aimé C., Gusachenko I., Kowalczuk L., Latour G., Coradin T., Schanne-Klein M.C.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
09/2014
Peer-reviewed
Oui
Volume
5
Pages
4920
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: epublish
Résumé
The quantification of collagen fibril size is a major issue for the investigation of pathological disorders associated with structural defects of the extracellular matrix. Second-harmonic generation microscopy is a powerful technique to characterize the macromolecular organization of collagen in unstained biological tissues. Nevertheless, due to the complex coherent building of this nonlinear optical signal, it has never been used to measure fibril diameter so far. Here we report absolute measurements of second-harmonic signals from isolated fibrils down to 30 nm diameter, via implementation of correlative second-harmonic-electron microscopy. Moreover, using analytical and numerical calculations, we demonstrate that the high sensitivity of this technique originates from the parallel alignment of collagen triple helices within fibrils and the subsequent constructive interferences of second-harmonic radiations. Finally, we use these absolute measurements as a calibration for ex vivo quantification of fibril diameter in the Descemet's membrane of a diabetic rat cornea.
Mots-clé
Animals, Collagen Type I/isolation & purification, Collagen Type I/ultrastructure, Descemet Membrane/pathology, Descemet Membrane/ultrastructure, Diabetes Mellitus, Experimental/pathology, Extracellular Matrix/chemistry, Extracellular Matrix/ultrastructure, Image Processing, Computer-Assisted/statistics & numerical data, Microscopy, Electron/instrumentation, Microscopy, Electron/methods, Rats, Tail/chemistry
Pubmed
Web of science
Création de la notice
01/03/2016 17:48
Dernière modification de la notice
03/03/2018 14:34
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